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Addgene

pMP472
(Plasmid #134997)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134997 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS
  • Backbone manufacturer
    Inder Verma
  • Total vector size (bp) 9591
  • Vector type
    Mammalian Expression, AAV, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    ** Be aware of whether you grow the reporter in dam- or dam+ bacterial strains (i.e., whether the basal state of the reporter will be fully methylated or unmethylated at GATC sites), especially for transient transfections. We use E. coli strain K12 ER2925 for growth for dam- background**
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Inters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
  • Species
    Synthetic
  • Insert Size (bp)
    8298

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMP472 was a gift from Ahmad Khalil (Addgene plasmid # 134997 ; http://n2t.net/addgene:134997 ; RRID:Addgene_134997)

  • For your References section:

    Engineering Epigenetic Regulation Using Synthetic Read-Write Modules. Park M, Patel N, Keung AJ, Khalil AS. Cell. 2019 Jan 10;176(1-2):227-238.e20. doi: 10.1016/j.cell.2018.11.002. Epub 2018 Dec 6. 10.1016/j.cell.2018.11.002 PubMed 30528434
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