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Addgene

pMP416
(Plasmid #134996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134996 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSP2638
  • Backbone manufacturer
    Keith Joung Lab
  • Total vector size (bp) 9990
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • dCas9 (N terminal on insert)
    • 3xFLAG
    • E coli Dam(N132A)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMP416 was a gift from Ahmad Khalil (Addgene plasmid # 134996 ; http://n2t.net/addgene:134996 ; RRID:Addgene_134996)

  • For your References section:

    Engineering Epigenetic Regulation Using Synthetic Read-Write Modules. Park M, Patel N, Keung AJ, Khalil AS. Cell. 2019 Jan 10;176(1-2):227-238.e20. doi: 10.1016/j.cell.2018.11.002. Epub 2018 Dec 6. 10.1016/j.cell.2018.11.002 PubMed 30528434
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