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Addgene

pMP315
(Plasmid #134994)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134994 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS
  • Backbone manufacturer
    Inder Verma
  • Total vector size (bp) 4988
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pUBC-KRAB-NLS-DpnI(aa146-254)
  • Species
    H. sapiens (human), Synthetic
  • Tags / Fusion Proteins
    • KRAB domain (N terminal on insert)
    • DpnI(aa146-254) (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMP315 was a gift from Ahmad Khalil (Addgene plasmid # 134994 ; http://n2t.net/addgene:134994 ; RRID:Addgene_134994)

  • For your References section:

    Engineering Epigenetic Regulation Using Synthetic Read-Write Modules. Park M, Patel N, Keung AJ, Khalil AS. Cell. 2019 Jan 10;176(1-2):227-238.e20. doi: 10.1016/j.cell.2018.11.002. Epub 2018 Dec 6. 10.1016/j.cell.2018.11.002 PubMed 30528434
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