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Addgene

CS2 luc MutB
(Plasmid #13499)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13499 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+luc
  • Backbone manufacturer
    See Rupp et al (1994) Genes Dev 8:1311
  • Backbone size w/o insert (bp) 5747
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fstl1
  • Alt name
    Follistatin-like-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    147
  • Mutation
    contains 3 tandem copies of the following sequence from the 3'UTR of Fstl1. Contains 8bp point mutation: 5'-acactgagtaccagagggcaagcccGTAGAATCcttctgctac-3'
  • GenBank ID
    NM_008047
  • Entrez Gene
    Fstl1 (a.k.a. AI316791, AW107808, Fstl, TSC-36)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer 5'-cataaaggccaagaagggcg-3' (in luciferase near BglII site)
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for picture of empty pCS2-luc.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CS2 luc MutB was a gift from Stephen Tapscott (Addgene plasmid # 13499 ; http://n2t.net/addgene:13499 ; RRID:Addgene_13499)

  • For your References section:

    MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206. Rosenberg MI, Georges SA, Asawachaicharn A, Analau E, Tapscott SJ. J Cell Biol. 2006 Oct 9. 175(1):77-85. 10.1083/jcb.200603039 PubMed 17030984
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