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pBABE MDproER
(Plasmid #13495)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13495 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    Morgenstern&Land (NAR 18:3587); Available at Addgene
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MyoD1-Estrogen Receptor Fusion Protein
  • Alt name
    Mouse Myod1
  • Alt name
    ESR1
  • Alt name
    Myod1
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    2700
  • Mutation
    full length MyoD (containing the point mutation A114P) was fused to aa282-595 of Esr1. Esr1 is inserted between codons 173 and 174 of MyoD.
  • GenBank ID
    NM_010866
  • Entrez Gene
    Myod1 (a.k.a. MYF3, MyoD, Myod-1, bHLHc1)
  • Entrez Gene
    ESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Entrez ID for MyoD is NM_010866 ; the Entrez ID for ER is NM_000125. See pBABE MDER for map of wild-type plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE MDproER was a gift from Stephen Tapscott (Addgene plasmid # 13495 ; http://n2t.net/addgene:13495 ; RRID:Addgene_13495)

  • For your References section:

    MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206. Rosenberg MI, Georges SA, Asawachaicharn A, Analau E, Tapscott SJ. J Cell Biol. 2006 Oct 9. 175(1):77-85. 10.1083/jcb.200603039 PubMed 17030984
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