pBABE MDER
(Plasmid #13494)

• Depositing Lab: Stephen Tapscott
• Publication: Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90(17):8028-32.

Backbone

• Vector backbone: pBABE puro
• Backbone manufacturer: Morgenstern&Land (NAR 18:3587); Available at Addgene
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MyoD1-Estrogen Receptor Fusion Protein
• Alt name: Mouse Myod1
• Alt name: Human Estrogen receptor 1 alpha
• Alt name: Myod1
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 2700
• Mutation: full length MyoD was fused to aa282-595 of Esr1. Esr1 is inserted between codons 173 and 174 of MyoD.
• GenBank ID: NM_010866
• Entrez Gene: Myod1 (a.k.a. MYF3, MyoD, Myod-1, bHLHc1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Harold Weintraub Lab, Fred Hutchinson Cancer Research Center
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Entrez ID for MyoD is NM_010866 ; the Entrez ID for ER is NM_000125.