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Addgene

GEF-H1 FLARE191.pa
(Plasmid #134931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134931 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEx-4
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5238
  • Total vector size (bp) 10000
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
  • Alt name
    GEF-H1 Flare 5.pa
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4578
  • Promoter CMV and T7
  • Tag / Fusion Protein
    • None.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer T7 Term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GEF-H1 FLARE191.pa was a gift from Klaus Hahn (Addgene plasmid # 134931 ; http://n2t.net/addgene:134931 ; RRID:Addgene_134931)

  • For your References section:

    Spatiotemporal dynamics of GEF-H1 activation controlled by microtubule- and Src-mediated pathways. Azoitei ML, Noh J, Marston DJ, Roudot P, Marshall CB, Daugird TA, Lisanza SL, Sandi MJ, Ikura M, Sondek J, Rottapel R, Hahn KM, Danuser G. J Cell Biol. 2019 Sep 2;218(9):3077-3097. doi: 10.1083/jcb.201812073. Epub 2019 Aug 16. 10.1083/jcb.201812073 PubMed 31420453
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