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Addgene

pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA Notes

Addgene Diagnostic Digest

This gel depicts the NEB extended 1kb ladder and a digestion of the plasmid pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA with the following enzymes: Pacl and Sall-HF.

Expected Bands:
PacI - 10.3 kb
SalI-HF - 10.3 kb
PacI/SalI-HF - 4.2 and 6.1 kb

We observed the expected bands.