GFP-Myo 1e
(Plasmid #134905)

• Purpose: Expression of rat Myo 1e (Myr 3) in mammalian cells.
• Depositing Lab: Martin Bähler
• Publication: Stoffler et al J Cell Biol. 1995 May;129(3):819-30. doi: 10.1083/jcb.129.3.819.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4696
• Total vector size (bp): 8162
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Myo 1e (rat) with 3' UTR
• Alt name: Myr 3
• Species: R. norvegicus (rat)
• Insert Size (bp): 3466
• GenBank ID: X74815
• Entrez Gene: Myo1e (a.k.a. MYR5, Myr3)
• Promoter: CMV IE
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Apa I (not destroyed)
• 5′ sequencing primer: EGFP-C CATGGTCCTGCTGGAGTTCGTG
• 3′ sequencing primer: SV40pA-R GAAATTTGTGATGCTATTGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry