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Addgene

pCAT219
(Plasmid #134887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBBR1MCS-2
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mRFP1
  • Species
    Synthetic
  • Insert Size (bp)
    922

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid parts were assembled using Golden gate method

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAT219 was a gift from Thomas Howard (Addgene plasmid # 134887 ; http://n2t.net/addgene:134887 ; RRID:Addgene_134887)

  • For your References section:

    pCAT vectors overcome inefficient electroporation of Cupriavidus necator H16. Azubuike CC, Gatehouse AMR, Howard TP. N Biotechnol. 2021 Nov 25;65:20-30. doi: 10.1016/j.nbt.2021.07.003. Epub 2021 Jul 29. 10.1016/j.nbt.2021.07.003 PubMed 34333160
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