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Addgene

pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin
(Plasmid #134869)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-EF1a-IRES-Puromycin
  • Backbone size w/o insert (bp) 8825
  • Total vector size (bp) 9655
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cytochrome b5 type A
  • Alt name
    CYB5A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    105
  • Mutation
    deleted amino acids 1-98
  • GenBank ID
    NM_001190807
  • Entrez Gene
    CYB5A (a.k.a. CYB5, MCB5, METAG)
  • Promoter EF1a
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer aag cgc gat cac atg gtc ctg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-EF1a-EGFP-Cybt5-IRES-Puromycin was a gift from David Andrews (Addgene plasmid # 134869 ; http://n2t.net/addgene:134869 ; RRID:Addgene_134869)

  • For your References section:

    A reference library for assigning protein subcellular localizations by image-based machine learning. Schormann W, Hariharan S, Andrews DW. J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 10.1083/jcb.201904090. 10.1083/jcb.201904090 PubMed 31968357
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