pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin
(Plasmid #134868)

• Purpose: Expresses EGFP-tagged lysosomal marker
• Depositing Lab: David Andrews
• Publication: Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 10.1083/jcb.201904090.

Backbone

• Vector backbone: pLVX-EF1a-IRES-Puromycin
• Backbone size w/o insert (bp): 8825
• Total vector size (bp): 10862
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lysosomal-associated membrane protein 1
• Alt name: LAMP-1
• Species: H. sapiens (human)
• Insert Size (bp): 1251
• Entrez Gene: LAMP1 (a.k.a. CD107a, LAMPA, LGP120)
• Promoter: EF1a
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: ATGCGATGGAGTTTCCCCAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: LAMP-1-GFP subcloned from Addgene #34831
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry