pLVX-EF1a-GFP-LaminA-IRES-Hygromycin
(Plasmid #134867)

• Purpose: Expresses EGFP-tagged nuclear envelope marker
• Depositing Lab: David Andrews
• Publication: Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 10.1083/jcb.201904090.

Backbone

• Vector backbone: pLVX-EF1a-IRES-Hygromycin
• Backbone size w/o insert (bp): 9258
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lamin A
• Species: H. sapiens (human)
• Insert Size (bp): 1995
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Promoter: EF1a
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: aag cgc gat cac atg gtc ctg

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GFP-LaminA subcloned from Addgene #17662
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry