pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin
(Plasmid
#134866)
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PurposeExpresses EGFP-tagged mutant of VAMP1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 134866 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX-EF1a-IRES-Hygromycin
- Backbone size w/o insert (bp) 9258
- Total vector size (bp) 10293
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVesicle-associated membrane protein 1
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Alt nameSynaptobrevin 1
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SpeciesH. sapiens (human)
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Insert Size (bp)289
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Mutationdeleted amino acids 97-118
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Entrez GeneVAMP1 (a.k.a. CMS25, SAX1, SPAX1, SYB1, VAMP-1)
- Promoter EF1a
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer aag cgc gat cac atg gtc ctg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-EF1a-delta-TMD-VAMP1-IRES-Hygromycin was a gift from David Andrews (Addgene plasmid # 134866 ; http://n2t.net/addgene:134866 ; RRID:Addgene_134866) -
For your References section:
A reference library for assigning protein subcellular localizations by image-based machine learning. Schormann W, Hariharan S, Andrews DW. J Cell Biol. 2020 Mar 2;219(3). pii: 133635. doi: 10.1083/jcb.201904090. 10.1083/jcb.201904090 PubMed 31968357