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Addgene

pAK_EF1a_CasRx_Hygro
(Plasmid #134840)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134840 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pXR001: EF1a-CasRx-2A-EGFP
  • Backbone manufacturer
    Patrick Hsu Lab
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RfxCas13d
  • Alt name
    CasRx
  • Insert Size (bp)
    2898
  • Promoter EF1a
  • Tag / Fusion Protein
    • T2A Hygromycin (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Patrick Hsu

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAK_EF1a_CasRx_Hygro was a gift from Michael Yaffe (Addgene plasmid # 134840 ; http://n2t.net/addgene:134840 ; RRID:Addgene_134840)
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