pHRdSV40-UP-24xGCN4-2xIFP2-CAAX-24xPP7
(Plasmid
#134833)
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PurposeExpresses 24xSunTag-2xIFP2-CAAX (24xGCN4_v4-2xIFP2-CAAX)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 134833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHRdSV40-UP
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert name24xGCN4_v4-2xIFP2-CAAX
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Alt name24xSunTag-2xIFP2-CAAX
- Promoter dSV40
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer dSV40 Forward Primer (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains a N7D mutation in the 23rd GCN4_v4 tag. This mutation is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHRdSV40-UP-24xGCN4-2xIFP2-CAAX-24xPP7 was a gift from Marvin Tanenbaum (Addgene plasmid # 134833 ; http://n2t.net/addgene:134833 ; RRID:Addgene_134833) -
For your References section:
Dynamics of Translation of Single mRNA Molecules In Vivo. Yan X, Hoek TA, Vale RD, Tanenbaum ME. Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034. 10.1016/j.cell.2016.04.034 PubMed 27153498