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Addgene

mSin1 shRNA #1
(Plasmid #13483)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13483 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1
  • Backbone size w/o insert (bp) 7032
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mSin1
  • Alt name
    shRNA mSin1
  • Alt name
    MAPKAP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    60
  • GenBank ID
    NM_024117
  • Entrez Gene
    MAPKAP1 (a.k.a. JC310, MIP1, SIN1, SIN1b, SIN1g)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LKO.1 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Short hairpin construct specific for human mSin1 (mSin1.1#1, TRC shRNA NM_024117.x-526s1c1) from the RNAi Consortium. The target sequence is 5'-GTTGG GACTT TGGTA TTAGA A-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mSin1 shRNA #1 was a gift from David Sabatini (Addgene plasmid # 13483 ; http://n2t.net/addgene:13483 ; RRID:Addgene_13483)

  • For your References section:

    mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM. Curr Biol. 2006 Aug 15. ():. 10.1016/j.cub.2006.08.001 PubMed 16919458
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