pED17x11
(Plasmid
#134814)
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PurposesfGFP expression plasmid for characterizing qtRNA charging
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 134814 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneKanR pUC
- Backbone size w/o insert (bp) 2429
- Total vector size (bp) 3171
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP-151-TAGA
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Insert Size (bp)742
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Mutation151-TAGA
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pED17x11 was a gift from Ahmed Badran (Addgene plasmid # 134814 ; http://n2t.net/addgene:134814 ; RRID:Addgene_134814) -
For your References section:
Multiplex suppression of four quadruplet codons via tRNA directed evolution. DeBenedictis EA, Carver GD, Chung CZ, Soll D, Badran AH. Nat Commun. 2021 Sep 29;12(1):5706. doi: 10.1038/s41467-021-25948-y. 10.1038/s41467-021-25948-y PubMed 34588441