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Addgene

pED14xW-1
(Plasmid #134805)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134805 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RK2 SpecR
  • Total vector size (bp) 3240
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow in glucose to suppress expression of qtRNA
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    W-TAGA qtRNA
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pED14xW-1 was a gift from Ahmed Badran (Addgene plasmid # 134805 ; http://n2t.net/addgene:134805 ; RRID:Addgene_134805)

  • For your References section:

    Multiplex suppression of four quadruplet codons via tRNA directed evolution. DeBenedictis EA, Carver GD, Chung CZ, Soll D, Badran AH. Nat Commun. 2021 Sep 29;12(1):5706. doi: 10.1038/s41467-021-25948-y. 10.1038/s41467-021-25948-y PubMed 34588441
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