pED14xS-4
(Plasmid
#134802)
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PurposeIPTG inducible expression of S-TAGA-Evo3 qtRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 134802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRK2 SpecR
- Total vector size (bp) 3240
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsGrow in glucose to suppress expression of qtRNA
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameS-TAGA-Evo3 qtRNA
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The lacI repressor must also be expressed in order to make the ProK-LacI promoter IPTG-inducible. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode lacI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pED14xS-4 was a gift from Ahmed Badran (Addgene plasmid # 134802 ; http://n2t.net/addgene:134802 ; RRID:Addgene_134802) -
For your References section:
Multiplex suppression of four quadruplet codons via tRNA directed evolution. DeBenedictis EA, Carver GD, Chung CZ, Soll D, Badran AH. Nat Commun. 2021 Sep 29;12(1):5706. doi: 10.1038/s41467-021-25948-y. 10.1038/s41467-021-25948-y PubMed 34588441