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Addgene

pED1a20
(Plasmid #134788)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134788 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    KanR SC101
  • Backbone size w/o insert (bp) 3330
  • Total vector size (bp) 5455
  • Modifications to backbone
    RepA-E93R mutation; copy # ~270 copies/cell.
  • Vector type
    Bacterial Expression, Luciferase, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    luxAB-357-CAGG
  • Insert Size (bp)
    2125
  • Mutation
    357-CAGG

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Genes luxCDE are also required to generate the luciferase substrate. These can be provided by the host strain, or on another plasmid. For example, E coli S2060s (Addgene Bacterial strain #105064), encode luxCDE.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pED1a20 was a gift from Ahmed Badran (Addgene plasmid # 134788 ; http://n2t.net/addgene:134788 ; RRID:Addgene_134788)

  • For your References section:

    Multiplex suppression of four quadruplet codons via tRNA directed evolution. DeBenedictis EA, Carver GD, Chung CZ, Soll D, Badran AH. Nat Commun. 2021 Sep 29;12(1):5706. doi: 10.1038/s41467-021-25948-y. 10.1038/s41467-021-25948-y PubMed 34588441
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