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Addgene

Lentiviral-His-tagged-PSPH
(Plasmid #134786)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134786 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-CMV-MCS-EF1-Puro
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 7377
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phosphoserine phosphatase
  • Alt name
    PSPH
  • Species
    H. sapiens (human)
  • Entrez Gene
    PSPH (a.k.a. PSP, PSPHD)
  • Promoter CMV
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lentiviral-His-tagged-PSPH was a gift from Seon-Young Kim (Addgene plasmid # 134786 ; http://n2t.net/addgene:134786 ; RRID:Addgene_134786)

  • For your References section:

    Phosphoserine Phosphatase Promotes Lung Cancer Progression through the Dephosphorylation of IRS-1 and a Noncanonical L-Serine-Independent Pathway. Park SM, Seo EH, Bae DH, Kim SS, Kim J, Lin W, Kim KH, Park JB, Kim YS, Yin J, Kim SY. Mol Cells. 2019 Aug 31;42(8):604-616. doi: 10.14348/molcells.2019.0160. 10.14348/molcells.2019.0160 PubMed 31446747
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