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pWay21-MKP-1L16A/L17A
(Plasmid #13478)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13478 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWay21 EGFP
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MKP-1L16A/L17A
  • Alt name
    MKP-1 FLL16A/L17A
  • Alt name
    MKP-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1130
  • Mutation
    Changed Leucine 16 to Alanine; changed Leucine 17 to Alanine
  • GenBank ID
    NM_013642
  • Entrez Gene
    Dusp1 (a.k.a. 3CH134, MKP1, Ptpn16, erp, mkp-1)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sma I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWay21-MKP-1L16A/L17A was a gift from Anton Bennett (Addgene plasmid # 13478 ; http://n2t.net/addgene:13478 ; RRID:Addgene_13478)

  • For your References section:

    The noncatalytic amino terminus of mitogen-activated protein kinase phosphatase 1 directs nuclear targeting and serum response element transcriptional regulation. Wu JJ, Zhang L, Bennett AM. Mol Cell Biol. 2005 Jun . 25(11):4792-803. 10.1128/MCB.25.11.4792-4803.2005 PubMed 15899879
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