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M3-M14-dCas9
(Plasmid #134779)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134779 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV
  • Backbone size w/o insert (bp) 3699
  • Total vector size (bp) 9420
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    METTL3/METTL14 methyltransferase domain and inactive dCas9
  • Species
    H. sapiens (human); S. pyogenes
  • Insert Size (bp)
    5721
  • GenBank ID
    NM_019852.5 NM_020961.4
  • Entrez Gene
    METTL3 (a.k.a. IME4, M6A, MT-A70, Spo8, hMETTL3)
  • Entrez Gene
    METTL14 (a.k.a. hMETTL14)
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer M3F, TAATACGACTCACTATAGGGAGAGCCGCCACCATGTTCCCACCTCAG; M14F, AGGTGGCTCCGGAGGGTCTGGTGGATCGGGAACACAGAGC
  • 3′ sequencing primer M3R, CAGACCCTCCGGAGCCACCTGATCCGCCTAAATTCTTAGG; M14R, GTGGCGGACTCTGAGGTCCCGGGAGTCTCGCTGCCGCTTCGGTCAGATTT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M3-M14-dCas9 was a gift from Shu-Bing Qian (Addgene plasmid # 134779 ; http://n2t.net/addgene:134779 ; RRID:Addgene_134779)

  • For your References section:

    Programmable RNA N(6)-methyladenosine editing by CRISPR-Cas9 conjugates. Liu XM, Zhou J, Mao Y, Ji Q, Qian SB. Nat Chem Biol. 2019 Sep;15(9):865-871. doi: 10.1038/s41589-019-0327-1. Epub 2019 Aug 5. 10.1038/s41589-019-0327-1 PubMed 31383972
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