pTF102
(Plasmid
#134774)
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Purpose(Empty Backbone) A binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134774 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPZP200
- Backbone size (bp) 6742
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Vector typePlant Expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains the 2099bp beta-glucuronidase (gus) containing an IV2 intron from E.coli
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTF102 was a gift from Kan Wang (Addgene plasmid # 134774 ; http://n2t.net/addgene:134774 ; RRID:Addgene_134774) -
For your References section:
Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system. Frame BR, Shou H, Chikwamba RK, Zhang Z, Xiang C, Fonger TM, Pegg SE, Li B, Nettleton DS, Pei D, Wang K. Plant Physiol. 2002 May;129(1):13-22. doi: 10.1104/pp.000653. 10.1104/pp.000653 PubMed 12011333
