pTF101.2
(Plasmid
#134771)
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Purpose(Empty Backbone) A binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 134771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPZP200
- Backbone size (bp) 6742
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Vector typePlant Expression
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains the 564bp bialaphos resistance (bar) gene from Streptomyces hygroscopicus
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTF101.2 was a gift from Kan Wang (Addgene plasmid # 134771 ; http://n2t.net/addgene:134771 ; RRID:Addgene_134771) -
For your References section:
Comparison of transformation frequency using the bar gene regulated by the CaMV 35S or NOS promoter in Agrobacterium-mediated soybean (Glycine max L.) transformation. Testroet A, Lee K, Luth D, Wang K. In Vitro Cell.Dev.Biol.-Plant (2017) 53: 188. 10.1007/s11627-017-9810-0 PubMed 16249869