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Addgene

pQXCINeo-LOX
(Plasmid #134761)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134761 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIN
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LOX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    -3
  • Entrez Gene
    LOX (a.k.a. AAT10)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer ACGCCATCCACGCTGTTTTGACCT
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PCR4-TOPO-LOX

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

(22pb before ATG and +46pb after STOP codon )5'-CTTAATTAACggATCCggTCAATCTggCAAAAgg-3' (5-prime-cloning primer); 5'-ggggggggCggAATTCAgAACACCAggCACTgAT-3' (3-prime-cloning primer)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQXCINeo-LOX was a gift from Philippe Clezardin & Martine Croset (Addgene plasmid # 134761 ; http://n2t.net/addgene:134761 ; RRID:Addgene_134761)
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