pWay21-MKP-1CH2A
(Plasmid
#13473)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13473 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepWay21 EGFP
- Backbone size w/o insert (bp) 5800
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMKP-1CH2A
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Alt nameMKP-1
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SpeciesM. musculus (mouse)
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Insert Size (bp)150
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MutationContains aa 1-46
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GenBank IDNM_013642
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Entrez GeneDusp1 (a.k.a. 3CH134, MKP1, Ptpn16, erp, mkp-1)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sma I (not destroyed)
- 3′ cloning site Not I (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWay21-MKP-1CH2A was a gift from Anton Bennett (Addgene plasmid # 13473 ; http://n2t.net/addgene:13473 ; RRID:Addgene_13473) -
For your References section:
The noncatalytic amino terminus of mitogen-activated protein kinase phosphatase 1 directs nuclear targeting and serum response element transcriptional regulation. Wu JJ, Zhang L, Bennett AM. Mol Cell Biol. 2005 Jun . 25(11):4792-803. 10.1128/MCB.25.11.4792-4803.2005 PubMed 15899879