pRcCMV-STAT31F
(Plasmid #134725)

• Purpose: Expresses chimeric STAT3-STAT1 protein with human STAT3 amino acids 1-514 joined to human STAT1 at amino acid 509, with a FLAG epitope tag at the C-terminus. Based on addgene plasmid #8691 and #8707
• Depositing Labs: Jim Darnell, Curt Horvath
• Publication: Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Backbone

• Vector backbone: pRcCMV
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7900
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STAT3-STAT1 Fusion
• Species: H. sapiens (human)
• Insert Size (bp): 2400
• Entrez Gene: STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
• Entrez Gene: STAT1 (a.k.a. CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91)
• Tags / Fusion Proteins:
  • human STAT3 aa 1-514 joined to human STAT1 at aa 509
  • FLAG (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry