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GC83 pComb3xss anti-Gephyrin nanobody
(Plasmid #134721)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134721 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pComb3xss
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-Gephyrin (Human) recombinant llama nanobody GC83
  • Species
    L. glama (llama)
  • Entrez Gene
    GPHN (a.k.a. GEPH, GPH, GPHRYN, HKPX1, MOCODC)
  • Promoter lac
  • Tag / Fusion Protein
    • 6xHis-HA (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (unknown if destroyed)
  • 3′ cloning site SfiI (unknown if destroyed)
  • 5′ sequencing primer 5’-TTAGGCACCCCAGGCTTTACACT-3’
  • 3′ sequencing primer 5’-GCCCCCTTATTAGCGTTTGCCATC-3’
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GC83 pComb3xss anti-Gephyrin nanobody was a gift from James Trimmer (Addgene plasmid # 134721 ; http://n2t.net/addgene:134721 ; RRID:Addgene_134721)

  • For your References section:

    A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in brain neurons. Dong JX, Lee Y, Kirmiz M, Palacio S, Dumitras C, Moreno CM, Sando R, Santana LF, Sudhof TC, Gong B, Murray KD, Trimmer JS. Elife. 2019 Sep 30;8. pii: 48750. doi: 10.7554/eLife.48750. 10.7554/eLife.48750 PubMed 31566565
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