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Addgene

p21-S208C/S242R/M694V-MEFV
(Plasmid #134706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PInducer21
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MEFV
  • Species
    H. sapiens (human)
  • Mutation
    p.S208C/S242R/M694V
  • Entrez Gene
    MEFV (a.k.a. FMF, MEF, PAAND, TRIM20)
  • Promoter TRE2 (Dox-inducible)
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p21-S208C/S242R/M694V-MEFV was a gift from Thomas Henry (Addgene plasmid # 134706 ; http://n2t.net/addgene:134706 ; RRID:Addgene_134706)

  • For your References section:

    Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients. Magnotti F, Lefeuvre L, Benezech S, Malsot T, Waeckel L, Martin A, Kerever S, Chirita D, Desjonqueres M, Duquesne A, Gerfaud-Valentin M, Laurent A, Seve P, Popoff MR, Walzer T, Belot A, Jamilloux Y, Henry T. EMBO Mol Med. 2019 Oct 7:e10547. doi: 10.15252/emmm.201910547. 10.15252/emmm.201910547 PubMed 31589380
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