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Addgene

pGEX-4T Doc2beta C2AB(125) H158C
(Plasmid #134691)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134691 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T
  • Backbone manufacturer
    GE Healthcare
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Double C2 protein beta
  • Species
    R. norvegicus (rat)
  • Mutation
    H158C, C145A, C217A, C249A, C290A, C338A, C387A
  • Entrez Gene
    Doc2b
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T Doc2beta C2AB(125) H158C was a gift from Edwin Chapman (Addgene plasmid # 134691 ; http://n2t.net/addgene:134691 ; RRID:Addgene_134691)

  • For your References section:

    Phosphatidylinositol 4,5-bisphosphate drives Ca(2+)-independent membrane penetration by the tandem C2 domain proteins synaptotagmin-1 and Doc2beta. Bradberry MM, Bao H, Lou X, Chapman ER. J Biol Chem. 2019 Jul 12;294(28):10942-10953. doi: 10.1074/jbc.RA119.007929. Epub 2019 May 30. 10.1074/jbc.RA119.007929 PubMed 31147445
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