pACYCDuet1-tglB
(Plasmid
#134676)
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PurposeExpresses His6-TglBin E. Coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 134676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYCDuet1
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Vector typeBacterial Expression
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Selectable markersChlR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTglB
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SpeciesPseudomonas syringae pv. maculicola str. ES4326
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Insert Size (bp)2448
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GenBank IDEGH61611.1
- Promoter T7
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Tag
/ Fusion Protein
- His6 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer ACYCDuetUP1
- 3′ sequencing primer DuetDOWN1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACYCDuet1-tglB was a gift from Wilfred van der Donk (Addgene plasmid # 134676 ; http://n2t.net/addgene:134676 ; RRID:Addgene_134676) -
For your References section:
Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products. Ting CP, Funk MA, Halaby SL, Zhang Z, Gonen T, van der Donk WA. Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232. 10.1126/science.aau6232 PubMed 31320540