pRSFDuet1-tglA(C51)HIB
(Plasmid
#134675)
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PurposeExpresses His6-TglA(C51), TglH, TglI and TglB in E. Coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134675 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSFDuet1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameTglA(C51)
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SpeciesPseudomonas syringae pv. maculicola str. ES4326
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Insert Size (bp)153
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Mutationinsertion of cysteine 51
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GenBank IDEGH61608.1
- Promoter T7
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Tag
/ Fusion Protein
- His6 (N terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer ACYCDuetUP1
- 3′ sequencing primer DuetDOWN1 (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameTglH
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SpeciesPseudomonas syringae pv. maculicola str. ES4326
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Insert Size (bp)916
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GenBank IDEGH61609.1
- Promoter T7
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Tag
/ Fusion Protein
- His6 (N terminal on insert)
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer ACYCDuetUP1
- 3′ sequencing primer DuetDOWN1 (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert nameTglI
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SpeciesPseudomonas syringae pv. maculicola str. ES4326
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Insert Size (bp)810
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GenBank IDEGH61610.1
- Promoter T7
Cloning Information for Gene/Insert 3
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer ACYCDuetUP1
- 3′ sequencing primer DuetDOWN1 (Common Sequencing Primers)
Gene/Insert 4
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Gene/Insert nameTglB
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SpeciesPseudomonas syringae pv. maculicola str. ES4326
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Insert Size (bp)2448
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GenBank IDEGH61611.1
- Promoter T7
Cloning Information for Gene/Insert 4
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer ACYCDuetUP1
- 3′ sequencing primer DuetDOWN1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSFDuet1-tglA(C51)HIB was a gift from Wilfred van der Donk (Addgene plasmid # 134675 ; http://n2t.net/addgene:134675 ; RRID:Addgene_134675) -
For your References section:
Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products. Ting CP, Funk MA, Halaby SL, Zhang Z, Gonen T, van der Donk WA. Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232. 10.1126/science.aau6232 PubMed 31320540
