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pRSFDuet1-tglA(C51)HIB
(Plasmid #134675)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134675 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSFDuet1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    TglA(C51)
  • Species
    Pseudomonas syringae pv. maculicola str. ES4326
  • Insert Size (bp)
    153
  • Mutation
    insertion of cysteine 51
  • GenBank ID
    EGH61608.1
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer ACYCDuetUP1
  • 3′ sequencing primer DuetDOWN1
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    TglH
  • Species
    Pseudomonas syringae pv. maculicola str. ES4326
  • Insert Size (bp)
    916
  • GenBank ID
    EGH61609.1
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer ACYCDuetUP1
  • 3′ sequencing primer DuetDOWN1
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    TglI
  • Species
    Pseudomonas syringae pv. maculicola str. ES4326
  • Insert Size (bp)
    810
  • GenBank ID
    EGH61610.1
  • Promoter T7

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer ACYCDuetUP1
  • 3′ sequencing primer DuetDOWN1
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    TglB
  • Species
    Pseudomonas syringae pv. maculicola str. ES4326
  • Insert Size (bp)
    2448
  • GenBank ID
    EGH61611.1
  • Promoter T7

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer ACYCDuetUP1
  • 3′ sequencing primer DuetDOWN1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDuet1-tglA(C51)HIB was a gift from Wilfred van der Donk (Addgene plasmid # 134675 ; http://n2t.net/addgene:134675 ; RRID:Addgene_134675)

  • For your References section:

    Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products. Ting CP, Funk MA, Halaby SL, Zhang Z, Gonen T, van der Donk WA. Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232. 10.1126/science.aau6232 PubMed 31320540
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