pET15b-tglHI
(Plasmid
#134672)
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PurposeExpresses His6-TglH and TglI in E. Coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 134672 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET15b
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Vector typeBacterial Expression
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Selectable markersAmpR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameTglH
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SpeciesPseudomonas syringae pv. maculicola str. ES4326
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Insert Size (bp)915
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GenBank IDEGH61609.1
- Promoter T7
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Tag
/ Fusion Protein
- His6 (N terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer T7 promoter primer
- 3′ sequencing primer T7 terminating primer (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameTglI
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Insert Size (bp)810
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GenBank IDEGH61610.1
- Promoter T7
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer T7 promoter primer
- 3′ sequencing primer T7 terminating primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET15b-tglHI was a gift from Wilfred van der Donk (Addgene plasmid # 134672 ; http://n2t.net/addgene:134672 ; RRID:Addgene_134672) -
For your References section:
Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products. Ting CP, Funk MA, Halaby SL, Zhang Z, Gonen T, van der Donk WA. Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232. 10.1126/science.aau6232 PubMed 31320540