Skip to main content
Addgene

pVDM1001
(Plasmid #134660)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134660 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pWS3
  • Backbone manufacturer
    Willem van Schaik
  • Backbone size w/o insert (bp) 4172
  • Total vector size (bp) 4822
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Escherichia coli EC1000
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRISPR guide RNA array
  • Insert Size (bp)
    650

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer AAAACTCGAGCCACTCACCATGGGTACTGCAG
  • 3′ sequencing primer AAAAGAATTCAACGTTGGCGATTCGTTGGCGATTGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVDM1001 was a gift from Willem van Schaik (Addgene plasmid # 134660 ; http://n2t.net/addgene:134660 ; RRID:Addgene_134660)

  • For your References section:

    CRISPR-Cas9-mediated genome editing in vancomycin-resistant Enterococcus faecium. de Maat V, Stege PB, Dedden M, Hamer M, van Pijkeren JP, Willems RJL, van Schaik W. FEMS Microbiol Lett. 2019 Nov 1;366(22). pii: 5697197. doi: 10.1093/femsle/fnz256. 10.1093/femsle/fnz256 PubMed 31905238
Related items:
Commonly requested with: