pIRES-HMGA1
(Plasmid #13466)

• Depositing Lab: Edward Whang
• Publication: Liau et al Cancer Res. 2006 Dec 15. 66(24):11613-22.

Backbone

• Vector backbone: pIRESpuro3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HMGA1
• Species: H. sapiens (human)
• Insert Size (bp): 323
• GenBank ID: NM_145899.2
• Entrez Gene: HMGA1 (a.k.a. HMG-R, HMGA1A, HMGIY)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: IMAGE Consortium
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The HMGA1 coding sequence was PCR amplified from IMAGE clone 5399570 (Genbank accession no. BC063434) using gene-specific primers modified to include the appropriate restriction sites at their 5' end. The primers used were forward, 5'-TTTTGATATCATGAGTGAGTCGAGCTCGAAG-3' and backward, 5'-TTTTGAATTCTCACTGCTCCTCCTCCGAGGA-3'. Purified PCR products were digested with EcoRV and EcoRI before ligation into an EcoRV/EcoRI–digested pIRES-puro3 vector (Clontech, Palo Alto, CA).