PJQ49
(Plasmid #134657)

• Purpose: (Empty Backbone) Contains Promoter 3 with bsa1 cut sites for inserting sgRNA
• Depositing Labs: Jason Peters, Oren Rosenberg
• Publication: Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-19. doi: 10.1128/JB.00304-19.

Backbone

• Vector backbone: pJMP1237
• Backbone manufacturer: Addgene 119262
• Backbone size (bp): 10856
• Modifications to backbone: The inducible PBAD promoter was replaced with the constitutive Biobrick promoter
• Vector type: Bacterial Expression, CRISPR
• Promoter: Anderson BBa_J23115
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BW25141
• Copy number: Unknown

Resource Information

• Supplemental Documents:
  • pJQ49.gb
• A portion of this plasmid was derived from a plasmid made by: Biobrick registry & Addgene 119262

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The parent plasmid was used in this paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424567/#SD7

We obtained the new promoter from the Biobrick registry.