GST-E6AP C833A
(Plasmid
#13465)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13465 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2T
- Backbone size w/o insert (bp) 4948
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameE6-AP
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SpeciesH. sapiens (human)
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Insert Size (bp)2500
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MutationC833A; active site Cys mutated to encode Ala
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Entrez GeneUBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A, PIX1)
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Tag
/ Fusion Protein
- GST
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer pGEX-5' (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Peter Howley
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
In addition to C833A, there is also an L837V amino acid change. E6-AP is missing the first 22 amino acids of isoform 1 (protein starts with MEACT NEFCA).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-E6AP C833A was a gift from Michael Greenberg (Addgene plasmid # 13465 ; http://n2t.net/addgene:13465 ; RRID:Addgene_13465)