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Addgene

PJQ47
(Plasmid #134646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJMP1237
  • Backbone manufacturer
    Addgene 119262
  • Backbone size (bp) 10856
  • Modifications to backbone
    The inducible PBAD promoter was replaced with the constitutive Biobrick promoter
  • Vector type
    Bacterial Expression, CRISPR
  • Promoter Anderson BBa_J23117
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25141
  • Copy number
    Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Biobrick registry & Addgene 119262

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The parent plasmid was used in this paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424567/#SD7

We obtained the new promoter from the Biobrick registry.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PJQ47 was a gift from Jason Peters & Oren Rosenberg (Addgene plasmid # 134646 ; http://n2t.net/addgene:134646 ; RRID:Addgene_134646)

  • For your References section:

    Modulating pathogenesis with Mobile-CRISPRi. Qu J, Prasad NK, Yu MA, Chen S, Lyden A, Herrera N, Silvis MR, Crawford E, Looney MR, Peters JM, Rosenberg OS. J Bacteriol. 2019 Sep 3. pii: JB.00304-19. doi: 10.1128/JB.00304-19. 10.1128/JB.00304-19 PubMed 31481541
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