pBa.GFP-KIF16B tail 458-1317
(Plasmid #134624)

• Purpose: Mammalian expression of human KIF16B 458-1317
• Depositing Lab: Marvin Bentley
• Publication: Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.

Backbone

• Vector backbone: pBa-eGFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KIF16B
• Species: H. sapiens (human)
• Mutation: WT with deletion of the 457 N-terminal residues.
• Entrez Gene: KIF16B (a.k.a. C20orf23, KISC20ORF, SNX23)
• Promoter: Chicken Beta actin
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.

Addgene QC NGS identified a M1027T discrepancy relative to Genbank accession: NP_078980.3. The depositing lab has confirmed that the plasmid should function as described in their publication.