pBa.GFP-KIF17 tail 400-1038
(Plasmid
#134617)
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PurposeMammalian expression of mouse KIF1A 400-1038
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134617 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBa-eGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKIF17
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SpeciesM. musculus (mouse)
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MutationWT with deletion of the 399 N-terminal residues.
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Entrez GeneKif17 (a.k.a. 5930435E01Rik, AW492270, Kif17b, mKIAA1405)
- Promoter Chicken Beta actin
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Tag
/ Fusion Protein
- eGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBa.GFP-KIF17 tail 400-1038 was a gift from Marvin Bentley (Addgene plasmid # 134617 ; http://n2t.net/addgene:134617 ; RRID:Addgene_134617) -
For your References section:
A novel strategy to visualize vesicle-bound kinesins reveals the diversity of kinesin-mediated transport. Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M. Traffic. 2019 Aug 28. doi: 10.1111/tra.12692. 10.1111/tra.12692 PubMed 31461551
