pBa.GFP-KIF3B tail 391-747
(Plasmid #134615)

• Purpose: Mammalian expression of mouse KIF3B 391-747
• Depositing Lab: Marvin Bentley
• Publication: Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.

Backbone

• Vector backbone: pBa-eGFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KIF3B
• Species: M. musculus (mouse)
• Mutation: WT with deletion of the 390 N-terminal residues.
• Entrez Gene: Kif5b (a.k.a. Khc, Khcs, Kns1, Ukhc)
• Promoter: Chicken Beta actin
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination.

Addgene QC NGS identified an A643E discrepancy, relative to Genbank accession: NP_032470.3. The depositing laboratory has confirmed that the plasmid should function as described in their publication.