pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955
(Plasmid #134613)

• Purpose: Mammalian expression of rat KIF5C 378-955
• Depositing Lab: Marvin Bentley
• Publication: Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.

Backbone

• Vector backbone: pCAG
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: KIF5C
• Species: R. norvegicus (rat)
• Mutation: WT with deletion of the 377 N-terminal residues.
• Entrez Gene: Kif5c
• Promoter: Chicken Beta actin
• Tag / Fusion Protein:
  • Halo (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC NGS identifies sequence homology for the plasmid insert to amino acids 377-955 of Genbank accession: XP_032759158.1 with an E636Q mutation. The depositing lab has confirmed that the plasmid should function as described in their publication.