pEGFP-N1-Flag-humanRBM47
(Plasmid #134607)

• Purpose: Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.
• Depositing Lab: Silvestro Conticello
• Publication: Chieca et al bioRxiv 2021

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 3960
• Total vector size (bp): 5787
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Flag-humanRBM47
• Species: H. sapiens (human)
• Insert Size (bp): 1827
• Entrez Gene: RBM47 (a.k.a. NET18)
• Promoter: CMV
• Tag / Fusion Protein:
  • flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: TTTTGCTAGCGTCGACCATGGACT
• 3′ sequencing primer: TTTTGGTACCTCAGTATGTCTGGTAG

Resource Information

• Supplemental Documents:
  • pEGFP-N1-FlagRBM47.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry