pBlueScript-humanAPOBEC1
(Plasmid #134606)

• Purpose: Plasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activity
• Depositing Lab: Silvestro Conticello
• Publication: Chieca et al bioRxiv 2021

Backbone

• Vector backbone: pBlueScript
• Backbone size w/o insert (bp): 7168
• Total vector size (bp): 7879
• Modifications to backbone: Added puromycin resistance and beta actin promoter
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: humanAPOBEC1
• Species: H. sapiens (human)
• Insert Size (bp): 711
• Entrez Gene: APOBEC1 (a.k.a. APO1, APOBEC-1, BEDP, CDAR1, HEPR)
• Promoter: Beta actin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: BsrGI (not destroyed)
• 5′ sequencing primer: AAAGCTAGCATGACTTCTGAGAAAGGTCCT
• 3′ sequencing primer: AAATGTACAAGATCTCATCTCCAAGCCACAGAAGG

Resource Information

• Supplemental Documents:
  • pBuerstedde-human Apobec1.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry