VPR_dCas9
(Plasmid #134601)

• Purpose: Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker
• Depositing Lab: Lasse Ebdrup Pedersen
• Publication: Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-598. doi: 10.1002/bit.27199. Epub 2019 Nov 12.

Backbone

• Vector backbone: pJ204
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 10240
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VPR-dCas9-2A-ZsGreen-DR
• Species: Synthetic
• Insert Size (bp): 7449
• Promoter: CMV
• Tags / Fusion Proteins:
  • VPR (N terminal on insert)
  • ZsGreen1-DR (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: AGTCGGTGTGTTGACATTGATTATTGACTA
• 3′ sequencing primer: ACGCAAGTCCATAGAGCCCACCGCATCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: VPR was cloned from Addgene plasmid # 68496
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry