pHCKan-yibDp-cimA3.7
(Plasmid
#134595)
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PurposeLow Phosphate inducible mutant citramalate synthase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 134595 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTWIST-Kan-High Copy
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecimA3.7
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Alt nameCitramalate synthase
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MutationI47V, E114V, H126Q, T204A, L238S
- Promoter yibDp
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer TGTAAAACGACGGCCAGT
- 3′ sequencing primer CAGGAAACAGCTATGAC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byConstructed by Twist Biosciences
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/820787v2 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHCKan-yibDp-cimA3.7 was a gift from Michael Lynch (Addgene plasmid # 134595 ; http://n2t.net/addgene:134595 ; RRID:Addgene_134595) -
For your References section:
Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion. Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, Wang R, Lynch MD. Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440. 10.1002/bit.27440 PubMed 32441815