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pHCKan-yibDp-cimA3.7
(Plasmid #134595)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134595 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTWIST-Kan-High Copy
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cimA3.7
  • Alt name
    Citramalate synthase
  • Mutation
    I47V, E114V, H126Q, T204A, L238S
  • Promoter yibDp

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
  • 3′ sequencing primer CAGGAAACAGCTATGAC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Constructed by Twist Biosciences

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/820787v2 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHCKan-yibDp-cimA3.7 was a gift from Michael Lynch (Addgene plasmid # 134595 ; http://n2t.net/addgene:134595 ; RRID:Addgene_134595)

  • For your References section:

    Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion. Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, Wang R, Lynch MD. Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440. 10.1002/bit.27440 PubMed 32441815
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