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pET28a-DDX43-NT-G46D
(Plasmid #134588)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134588 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6052
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    None
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX43-N-terminal (1-253 aa) with G46D mutation
  • Alt name
    HAGE
  • Alt name
    DEAD-Box Helicase 43
  • Alt name
    CT13
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1944
  • Mutation
    full-length (G154D)
  • GenBank ID
    NM_018665
  • Entrez Gene
    DDX43 (a.k.a. CT13, HAGE)
  • Promoter T7
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site Xho I (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-DDX43-NT-G46D was a gift from Yuliang Wu (Addgene plasmid # 134588 ; http://n2t.net/addgene:134588 ; RRID:Addgene_134588)

  • For your References section:

    The DEAD-box protein DDX43 (HAGE) is a dual RNA-DNA helicase and has a K-homology domain required for full nucleic acid unwinding activity. Talwar T, Vidhyasagar V, Qing J, Guo M, Kariem A, Lu Y, Singh RS, Lukong KE, Wu Y. J Biol Chem. 2017 Jun 23;292(25):10429-10443. doi: 10.1074/jbc.M117.774950. Epub 2017 May 3. 10.1074/jbc.M117.774950 PubMed 28468824
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