YFP-PCAF-C2
(Plasmid
#134572)
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PurposeExpresses YFP-PCAF fusion protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134572 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEYFP-C2
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePCAF
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SpeciesM. musculus (mouse)
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Entrez GeneKat2b (a.k.a. A930006P13Rik, AI461839, AW536563, Pc, Pcaf, p/CAF)
- Promoter CMV
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Tag
/ Fusion Protein
- YFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindII (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YFP-PCAF-C2 was a gift from Susan Janicki (Addgene plasmid # 134572 ; http://n2t.net/addgene:134572 ; RRID:Addgene_134572) -
For your References section:
Single cell analysis of transcriptional activation dynamics. Rafalska-Metcalf IU, Powers SL, Joo LM, LeRoy G, Janicki SM. PLoS One. 2010 Apr 21;5(4):e10272. doi: 10.1371/journal.pone.0010272. 10.1371/journal.pone.0010272 PubMed 20422051
